The PKCα activity was detected using HTRF technology. The reaction was performed by incubating the PKCα protein, ATP and substrate at 25℃ for 60 min, then adding beads at 25℃ for 60 min and reading ratio 665/620 with BMG.
PKCα Protein
PKCα Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P17252 |
| Amino Acid Sequence | M1-V672(end) |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 102577.0 |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50mM Tris, 150mM NaCl, 1mM DTT, 0.1mM PMSF, pH7.5 |
| Stability & Storage | -80℃ |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg PKCα
2 3 μg PKCα
