1μg (R: reducing condition, N: non-reducing condition).
NCAM-1/CD56 His Tag Protein, Rat
NCAM-1/CD56 His Tag Protein, Rat
Price:
Regular price
$320 USD
Regular price
Sale price
$320 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species | Rat |
| Synonyms | N-CAM-1, NCAM-1, CD56, MSK39, NCAM1 |
| Accession | P13596-1 |
| Amino Acid Sequence | Leu20-Thr721, with C-terminal His |
| Expression System | HEK293 |
| Molecular Weight | 95-115kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & >95% by SEC-HPLC |
| Endotoxin | <0.1EU/μg |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, 5% trehalose, PH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Reyes AA. et al., 1991, Mol Cell Biol. 11 (3): 1654-61. |
Background
NCAM1 is a neural adhesion protein (NCAM) that belongs to the immunoglobulin superfamily. Human NCAM-1 is synthesized as a 761 aa preproprecursor, containing a signal sequence, a large GPI-linked extracellular domain (ECD), and a short C-terminal prosegment. The mature ECD contains five C-2 type Ig-like domains and two fibronectin type III domains. The mature human ECD shares 93% and 95% aa sequence identity with the mouse and rat NCAM-1 ECD, respectively. The encoded protein is involved in cell-to-cell interactions as well as cell-matrix interactions during development and differentiation. NCAM1 is involved in neuron-neuron adhesion, neurite fasciculation, the outgrowth of neurites, etc. It has also been shown to be involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance. Diseases associated with NCAM1 include Rabies and Bile Duct Cancer. Among its related pathways are Neuroscience and RET signaling.
Picture
Picture
SDS-PAGE
SEC-HPLC
The purity of NCAM-1/CD56 His Tag, Rat is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized NCAM-1/CD56 His Tag Protein, Rat (Cat. No. UA010958) at 2.0μg/mL (100μL/well) can bind NCAM /CD56 Rabbit mAb (A23300) with EC50 of 0.48-0.81ng/mL.
