2μg (R: reducing conditions, N: non-reducing conditions).
IL-18BP Fc Chimera Protein, Human
IL-18BP Fc Chimera Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | IL-18BP |
| Synonyms | IL18BP, Tadekinig-alfa |
| Accession | O95998-2 |
| Amino Acid Sequence | Thr31-Gly194, with C-terminal Human IgG1 Fc TPVSQTTTAATASVRSTKDPCPSQPPVFPAAKQCPALEVTWPEVEVPLNGTLSLSCVACSRFPNFSILYWLGNGSFIEHLPGRLWEGSTSRERGSTGTQLCKALVLEQLTPALHSTNFSCVLVDPEQVVQRHVVLAQLWAGLRATLPPTQEALPSSHSSPQQQGIEGRMDPKSSDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK |
| Expression System | HEK293 |
| Molecular Weight | 53-76kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human Fc |
| Physical Appearance | Lyophilized powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Review Front ImmunoQl . 2013 Oct 8;4:289. |
Background
Interleukin-18 binding protein, also known as IL-18BP and tadekinig-alfa, is a secreted glycoprotein that contains an Ig-like C2-type domain. IL-18BP is a cytokine receptor that belongs to the interleukin 1 receptor family. IL18BP shares many characteristics with soluble cytokine receptors of the IL1 family in that the protein exhibits specificity for IL18, belongs to the immunoglobulin-like class of receptors and has limited amino acid sequences with those of the IL1 receptor type II. According to the other study, IL1R9 is evolutionarily related to IL18BP and may function as an IL-18 receptor. Plasma il-18 /IL18BP levels increase during disease activity, suggesting that it may play a role in the pathogenesis of ITP.
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SDS-PAGE
ELISA
Measured by its binding ability in a functional ELISA. When Recombinant Human IL-18 is immobilized 2.5µg/ml (100µl/well), Recombinant Human IL-18BP Fc Chimera binds with an EC50 of 0.07-0.1μg/ml.
Immobilized IL-18, Human (Cat. No. UA040111)
at 2.0μg/mL (100μL/well) can bind IL-18BP Fc Chimera, Human (Cat. No. UA010587)
with EC50 of 2.20-3.25ng/ml.
SPR
Protein A Chip captured IL-18BP Fc Chimera, Human (Cat. No. UA010587), can bind IL-18, Human(Cat. No. UA040111) with an affinity constant of 75.59pM as determined in SPR assay.
