The GSPT1 activity was detected using HTRF technology. The reaction was performed by incubating the GSPT1 protein, CRBN and varying concentration of CC-885
and beads
at 25℃ for 60 min, then reading ratio 665/620 on BMG.
Human GSPT1 ,GST tag Protein
Human GSPT1 ,GST tag Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | ERF3A |
| Accession | P15170-3 |
| Amino Acid Sequence |
D437-V633
DGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLV |
| Expression System | E.coli |
| Molecular Weight | 48.9 kDa |
| Purity | >90% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | GST Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5 |
| Stability & Storage |
Stable for 12 months
upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws
cycles.
|
Picture
Picture
Bioactivity
SDS-PAGE
SEC-HPLC
