The GAK activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the GAK protein , ATP and substrate at 25℃ for 120 min, then adding ADP-Glo reagent and reading RLU with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Synonyms | GAK |
Accession | O14976 |
Amino Acid Sequence |
G12-P347
AGPGSLGGASGRDQSDFVGQTVELGELRLRVRRVLAEGGFAFVYEAQDVGSGREYALKRLLSNEEEKNRAIIQEVCFMKKLSGHPNIVQFCSAASIGKEESDTGQAEFLLLTELCKGQLVEFLKKMESRGPLSCDTVLKIFYQTCRAVQHMHRQKPPIIHRDLKVENLLLSNQGTIKLCDFGSATTISHYPDYSWSAQRRALVEEEITRNTTPMYRTPEIIDLYSNFPIGEKQDIWALGCILYLLCFRQHPFEDGAKLRIVNGKYSIPPHDTQYTVFHSLIRAMLQVNPEERLSIAEVVHQLQEIAAARNVNPKSPITELLEQNGGYGSATLSRGP |
Expression System | E.coli |
Molecular Weight | 39.8 kDa |
Purity | >95% by SDS-PAGE |
Endotoxin | <1EU/μg |
Conjugation | Unconjugated |
Tag | His Tag |
Physical Appearance | Liquid |
Storage Buffer | 20 mM Tris, 200 mM NaCl, 1 mM DTT, 10% glycerol, pH 7.5 |
Stability & Storage |
Stable for
12 months upon stored at -80℃ from the date of receipt. And avoid repeated
freeze-thaws cycles.
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Picture
Picture
Bioactivity


SDS-PAGE

SEC-HPLC




