2μg (R: reducing condition, N: non-reducing condition).
Ephrin-A1 Fc Chimera Protein, Human
Ephrin-A1 Fc Chimera Protein, Human
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$200 USD
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$200 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | EFNA1, LERK-1, TNFAIP4, EPLG1, B61, ECKLG, EFL1 |
| Accession | P20827-1 |
| Amino Acid Sequence | Asp19-Ser182, with hIgG Fc |
| Expression System | HEK293 |
| Molecular Weight | 51-55 kDa (R) |
| Purity | ＞95% by SDS-PAGE & RP-HPLC |
| Conjugation | Unconjugated |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Singh PK, Rybak JA, Schuck RJ, Barrera FN, Smith AW. Phosphatidylinositol (4,5)-bisphosphate drives the formation of EGFR and EphA2 complexes. bioRxiv [Preprint]. 2024 May 4:2024.05.03.592400. PMID: 38746348; |
Background
Ephrin-A1 (EFNA1) is also known as EPLG1, LERK1, TNFAIP4, Immediate early response protein B61, which belongs to the ephrin family. EFNA1 contains one ephrin RBD (ephrin receptor-binding) domain. EFNA1 is cell surface GPI-bound ligand for Eph receptors, and is a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development.
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SDS-PAGE
RP-HPLC
The purity of Ephrin-A1 Fc Chimera Protein, Human is greater than 95% as determined by RP-HPLC.
ELISA
Immobilized Biotinylated EphA2 His&Avi Tag Protein, Human (Cat. No. UA010833) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Ephrin-A1 Fc Chimera Protein, Human (Cat. No. UA011030) with EC50 of 1.20-1.74 ng/ml.
Immobilized EphA2 His Tag, Human (Cat. No. UA010580) at 2μg/mL (100μL/well) can bind Ephrin-A1 Fc Chimera Protein, Human (Cat. No. UA011030) with EC50 of 0.70-1.00 ng/ml.
