EPHA4 Protein

Catalog Number: UA080079 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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$995.00 SGD

Size: 10μg 100μg 1mg

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Product Details

Product Specification

Species	Human
Accession	P54764-1
Amino Acid Sequence	E586-V986(end) EKHLNQGVRTYVDPFTYEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREICVAIKTLKAGYTDKQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVIQLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDDPEAAYTTRGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGYRLPPPMDCPIALHQLMLDCWQKERSDRPKFGQIVNMLDKLIRNPNSLKRTGTESSRPNTALLDPSSPEFSAVVSVGDWLQAIKMDRYKDNFTAAGYTTLEAVVHVNQEDLARIGITAITHQNKILSSVQAMRTQMQQMHGRMVPV
Expression System	Baculovirus-InsectCells
Molecular Weight	71.9 kDa
Purity	>95% by SDS-PAGE
Endotoxin	<1EU/μg
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	50mM Tris, 150mM NaCl, 20mM GSH, 1mM DTT, 10% glycerol, 0.05% Brij-35, pH7.5
Stability & Storage	-80℃

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Bioactivity

EPHA4 titration in HTRF assay

The EPHA4 activity was detected using HTRF technology. The reaction was performed by incubating the EPHA4 protein , ATP and substrate at 25℃ for 50 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.