EPHA2 titration in HTRF assay
EPHA2 Protein
EPHA2 Protein
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P29317-1 |
| Amino Acid Sequence |
E572-I976(end) EDVYFSKSEQLKPLKTYVDPHTYEDPNQAVLKFTTEIHPSCVTRQKVIGAGEFGEVYKGMLKTSSGKKEVPVAIKTLKAGYTEKQRVDFLGEAGIMGQFSHHNIIRLEGVISKYKPMMIITEYMENGALDKFLREKDGEFSVLQLVGMLRGIAAGMKYLANMNYVHRDLAARNILVNSNLVCKVSDFGLSRVLEDDPEATYTTSGGKIPIRWTAPEAISYRKFTSASDVWSFGIVMWEVMTYGERPYWELSNHEVMKAINDGFRLPTPMDCPSAIYQLMMQCWQQERARRPKFADIVSILDKLIRAPDSLKTLADFDPRVSIRLPSTSGSEGVPFRTVSEWLESIKMQQYTEHFMAAGYTAIEKVVQMTNDDIKRIGVRLPGHQKRIAYSLLGLKDQVNTVGIPI |
| Expression System | Baculovirus-InsectCells |
| Molecular Weight | 72.5 kDa |
| Purity | >90% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 20mM Reduced Glu, 10% glycerol, 1mM DTT, pH 8.0 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
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Bioactivity
The EPHA2 activity was detected using homogeneous time-resolved fluorescence technology. The reaction was performed by incubating the EPHA2 protein , ATP and substrate at 25℃ for 50 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 nm with BMG.
SDS-PAGE
SEC-HPLC
