The EGFR[L858R/T790M] activity was detected using HTRF technology. The reaction was performed by incubating the EGFR[L858R/T790M] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Accession | P00533-1 |
Amino Acid Sequence | R669-A1210(end) |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 87.8 kDa |
Purity | >85% by SDS-PAGE |
Endotoxin | <1EU/μg |
Conjugation | Unconjugated |
Tag | GST Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Hepes, 150 mM NaCl, 20 mM Reduced Glu, 1 mM DTT, 10% Glycerol, pH7.5 |
Stability & Storage |
Stable for
12 months upon stored at -80℃ from the date of receipt. And avoid repeated
freeze-thaws cycles.
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Picture
Picture
Bioactivity


SDS-PAGE

M MW Standard
1 1 μg EGFR[L858R/T790M]
2 3 μg EGFR[L858R/T790M]


