The EGFR[C797S] activity was detected using HTRF technology. The reaction was performed by incubating the EGFR[C797S] protein, ATP and substrate at 25℃ for 60 min, adding the beads at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Product Details
Product Details
Product Specification
Species | Human |
Accession | P00533-1 |
Amino Acid Sequence | R669-A1210(end), C797S with N-terminal GST tag |
Expression System | Baculovirus-InsectCells |
Molecular Weight | 87.8 kDa |
Purity | >90% by SDS-PAGE |
Conjugation | Unconjugated |
Tag | GST Tag |
Physical Appearance | Liquid |
Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, 10% Glycerol, , 0.05% Brij35, pH7.5 |
Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
