Cullin2-RBX1-ElonginB/C-VHL Protein

Catalog Number: UA080274 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE

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Product Details

Product Specification

Species	Human
Synonyms	VHL/ELOB/ELOC/CUL2/RBX1 Complex
Amino Acid Sequence	His-RBX1 (full-length), HA-Cullin2 (full-length), HA-ElonginC (full-length), ElonginB (full-length), VHL (full-length)
Expression System	Sf9 and E. coli
Purity	＞90% by SDS-PAGE
Endotoxin	<1EU/μg
Conjugation	Unconjugated
Physical Appearance	Liquid
Storage Buffer	20mM HEPES, 200mM NaCl, 5% Glycerol, 2mM TCEP, pH 8.0
Stability & Storage	Store at -80ºC. Avoid multiple freeze-thaw cycles.

Background

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues. Targets of VHL include HIF1 alpha, beta 2 adrenergic receptor, ZHX2 and others. In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families. ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.

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Bioactivity

The Cullin2-RBX1-ElonginB/C-VHL activity was measured by protein-tracer binary binding assay using HTRFtechnology.

The Cullin2-RBX1-ElonginB/C-VHL activity was measured by PROTAC-mediated Ternary Complex Formation using HTRFtechnology. The dose response curve of ARV-771 in the Ternary Assay exhibited a bell-shaped curve, a hook effect and a peak.

The Cullin2-RBX1-ElonginB/C-VHL activity was measured by WB analysis for target protein ubiquitination assay. The Ubiquitinated BRD4(D1+D2) bands increased with time.