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Claudin-4 protein his tag (detergent, full length), Human

Claudin-4 protein his tag (detergent, full length), Human

Catalog Number: UA060001 Reactivity: Human Conjugation: Unconjugated Brand: UA BIOSCIENCE
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Regular price $1,400 USD
Regular price Sale price $1,400 USD
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Product Details

Product Specification


Species Human
Synonyms Clostridium perfringens enterotoxin receptor, CLDN4, CPE-R, hCPE-R, WBSCR8
Accession O14493
Concentration 0.65mg/ml by Nanodrop
Amino Acid Sequence

Wild type full length with his tag at C-Terminus

Expression System Baculovirus-InsectCells
Molecular Weight

23.9 kDa

Purity >95% by SDS-PAGE &>95% by SEC-HPLC
Endotoxin <0.1EU/μg
Conjugation Unconjugated
Tags & Cleavage sites Thrombin cleave site
Storage Buffer

20 mM Hepes, pH 8.0, 100 mM NaCl, 0.04 % DDM

Stability & Storage

·6 months from date of receipt, -60 to -80 °C as supplied.
·about 3 days in 2 to 8 °C.
·Please avoid repeated freeze-thaw cycles.

Reference

1. Vecchio, Alex J., Sewwandi S. Rathnayake, and Robert M. Stroud. "Structural basis for Clostridium perfringens enterotoxin targeting of claudins at tight junctions in mammalian gut." Proceedings of the National Academy of Sciences 118.15 (2021): e2024651118.

Background

Claudin 4/CLDN4 is a member of the Claudins family and is one membrane protein of the key Junctions for Tight Junctions. Claudins membrane proteins are composed of four transmembrane domains, and there is a binding domain at the C-terminal, which can bind to proteins in the inner cytoplasm (such as ZO-1), and play an important role in signal transduction. Outside the cell membrane, there are two rings of different lengths (ECL1 and ECL2), which can be in contact with other nearby rings of the same type, forming a tight connection between cells. The extracellular ring is necessary to maintain tight junction function and the integrity of the epithelial barrier. Tight junctions are intercellular adhesion structures located at the tips of epithelial and endothelial cells. Claudins, as an important component of tight junctions, play a role in regulating epithelial cell permeability and maintaining cell polarity. When Claudins protein mutated, the permeability between cells will be affected, which may lead to the occurrence of diseases. More and more studies have revealed that CLDN4 plays a regulatory role in cancer and may be a potential tumor therapeutic target. In fact, a large amount of evidence has shown that Claudins members play an important role in tumor immunotherapy and have great clinical druggability.

Picture

SDS-PAGE

1μg (R: reducing condition, N: non-reducing condition).

SEC-HPLC

The purity of Claudin4 Protein his tag thrombin site in detergent is greater than 90% as determined by SEC-HPLC.

ELISA

Immobilized Human Claudin-4 Antibody at 2.0μg/mL (100μL/well) can bind Claudin4 membrane Protein with his tag and thrombin site in detergent, Human (Cat. No. UA060001) with EC50 of 2.33-3.61ng/mL.

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