1μg (R: reducing condition, N: non-reducing condition).
CD9/Tspan-29 His Tag Protein, Human
CD9/Tspan-29 His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | MIC3, TSPAN29, GIG2, MRP1, BTCC1, DRAP27, 5H9 |
| Accession | P21926 |
| Amino Acid Sequence | Ser112-Ile195, with C-8*His |
| Expression System | HEK293 |
| Molecular Weight | 11-12kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35. Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12. |
Background
CD9 antigen is a multi-pass membrane protein which belongs to the tetraspanin (TM4SF) family or the transmembrane 4 superfamily. It is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It is found expressed on the surface of the exosomes. The protein takes part in cellular signal transduction events and thus play a role in the regulation of cell development and activation, growth and motility. Besides, CD9 seems to be a key role in the egg-sperm fusion during the mammalian fertilization processes.
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SDS-PAGE
ELISA
Immobilized CD9/Tspan-29 His Tag, Human (Cat. No. UA010729) at 2.0μg/mL (100μL/well) can bind CD9 Mouse mAb (S-632-33) (Cat. No. S0B0409) with EC50 of 1.69-2.96ng/mL.
