2μg (R: reducing condition, N: non-reducing condition).
CD55/DAF His Tag Protein, Mouse
CD55/DAF His Tag Protein, Mouse
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | CD55,DAF,CR,CROM,TC |
| Accession | Q61475 |
| Amino Acid Sequence | Asp35-T361, with C-10*His |
| Expression System | HEK293 |
| Molecular Weight | 45-72kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Journal List Immunology v.100(4); 2000 Aug PMC2327033. |
Background
CD55, also known as DAF or decay acceleration factor, is a 70-75 kDa member of the RCA protein family that is characterized by four to 30 SCRS (short consistent repeats) in its plasma exposure area. It prevents the assembly of the C3bBb complex (the C3-convertase of the alternative pathway) or accelerates the disassembly of preformed convertase, thus blocking the formation of the membrane attack complex. DAF seems to exert different functions beyond its immunological role such as promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility.
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