1μg (R: reducing conditions, N: non-reducing conditions).
CD39/ENTPD1 His Tag Protein, Human
CD39/ENTPD1 His Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | CD39/ENTPD1 |
| Synonyms | CD39, Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, NTPDase 1, Ecto-ATPDase 1 |
| Accession | P49961-1 |
| Amino Acid Sequence | Thr38 -Val478, with C-terminal 10*His TQNKALPENVKYGIVLDAGSSHTSLYIYKWPAEKENDTGVVHQVEECRVKGPGISKFVQKVNEIGIYLTDCMERAREVIPRSQHQETPVYLGATAGMRLLRMESEELADRVLDVVERSLSNYPFDFQGARIITGQEEGAYGWITINYLLGKFSQKTRWFSIVPYETNNQETFGALDLGGASTQVTFVPQNQTIESPDNALQFRLYGKDYNVYTHSFLCYGKDQALWQKLAKDIQVASNEILRDPCFHPGYKKVVNVSDLYKTPCTKRFEMTLPFQQFEIQGIGNYQQCHQSILELFNTSYCPYSQCAFNGIFLPPLQGDFGAFSAFYFVMKFLNLTSEKVSQEKVTEMMKKFCAQPWEEIKTSYAGVKEKYLSEYCFSGTYILSLLLQGYHFTADSWEHIHFIGKIQGSDAGWTLGYMLNLTNMIPAEQPLSTPLSHSTYVGGGSGGGSHHHHHHHHHH |
| Expression System | HEK293 |
| Molecular Weight | 70-80kDa |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Grinthal, A. and G. Guidotti. (2004) Biochemistry 43:13849. 2.Robson, S.C. et al. (2006) Purinergic Signal. 2:409. 3.Zebisch, M. et al. (2012) J. Mol. Biol. 415:288. |
Background
CD39, also known as ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1), is the prototypical member of the NTPDase family. CD39 is a metal-dependent, homodimeric enzyme bound to the cell membrane through two transmembrane domains present at each termini. CD39 hydrolyzes the beta - and gamma phosphate residues of nucleotides to terminate purinergic signaling and is uniquely capable of processive hydrolysis of extracellular ATP to AMP without release of the ADP intermediate as the initial rate-limiting steps in the production of extracellular adenosine. CD39 ecto-nucleotidase is the rate-limiting enzyme of a cascade leading to the generation of suppressive adenosine that alters CD4 and CD8 T cell and natural killer cell antitumor activities.
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SDS-PAGE
ELISA
Immobilized CD39/ENTPD1 His Tag Protein, Human (Cat. No. UA010585) at 2.0μg/mL (100μL/well) can bind CD39 Recombinant Rabbit mAb (SDT-236-20) (Cat. No. S0B0050) with EC50 of 0.76-1.17ng/mL.
