Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The EC50 for this effect is typically 9-25ng/ml.
Biotinylated Transferrin His&Avi Tag Protein, Human
Biotinylated Transferrin His&Avi Tag Protein, Human
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$560 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Beta-1 metal-binding globulin，Siderophilin，Serotransferrin，TF，TRF，DKFZp781D0156, PRO1557, PRO2086，HEL-S-71p，TFQTL1 |
| Accession | P02787 |
| Amino Acid Sequence | Val20-Pro698 with His & Avi Tag at the C-Terminus |
| Expression System | HEK293 |
| Molecular Weight | 70-85kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE & SEC-HPLC |
| Endotoxin | <1EU/μg |
| Conjugation | Biotin |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, PH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | Stearne P.A. et al. (1985) J. Immunol. 134:3474. |
Background
Transferrin is a glycoprotein with an approximate molecular weight of 76.5 kDa. It is believed to have originated from an ancient gene duplication event, resulting in homologous C and N-terminal domains that each bind one ferric ion. The primary function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to proliferating cells throughout the body. Additionally, transferrin may serve a physiological role as a granulocyte/pollens-binding protein (GPBP), which is involved in the clearance of certain organic materials and allergens from the serum.
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Bioactivity
SDS-PAGE
1μg (R: reducing condition, N: non-reducing condition).
SEC-HPLC
The purity of Biotinylated Transferrin His & Avi Tag Protein, Human is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized Biotinylated Transferrin His&Avi Tag Protein, Human (Cat. No. UA011027) at 2μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Human Transferrin R / CD71 Protein, Fc Tag with EC50 of 8.90-14.7ng/ml.
