2e5 of transient transfected anti-ICAM-1 ScFv CAR-293 cells were stained with 0. 1ug Biotinylated ICAM-1/CD54 His&Avi Tag Protein, Human, (Cat. No. UA010854) and unrelated protein (Fig. C and B), and non-transfected 293 cells were used as a control (Fig. A). PE signal was used to evaluate the binding activity.
2e5 of transient transfected anti-ICAM-1 ScFv CAR-293 cells were stained with 0. 1ug ICAM-1/CD54 His Tag Protein, Human, (Cat. No. UA010179) (Fig. D). APC signal was used to evaluate the binding activity.
Biotinylated ICAM-1/CD54 His&Avi Tag Protein, Human
Biotinylated ICAM-1/CD54 His&Avi Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | BB2, ICAM1, CD54, P3.58 |
| Accession | P05362-1 |
| Amino Acid Sequence | Gln28-Glu480, with C-terminal His and Avitag |
| Expression System | HEK293 |
| Molecular Weight | 72-90kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Theresa N. Ramos; Daniel C. Bullard; Scott R. Barnum:ICAM-1: Isoforms and Phenotypes, J Immunol (2014) 192 (10): 4469–4474. |
Background
ICAM-1 is a cell surface glycoprotein expressed at a low basal level in immune, endothelial (EC) and epithelial cells, but is up-regulated in response to inflammatory stimulation. The function of ICAM-1 has been best studied in leukocyte transendothelial migration (TEM), where ICAM-1 regulates leukocyte rolling and adhesive interactions with the vessel wall, and guides leukocyte crossing of the endothelial layer. More recently, functional studies identified several new roles of ICAM-1 in epithelial injury-resolution responses, innate and adaptive immune responses in inflammation, and tumorigenesis.
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SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
ELISA
Immobilized Biotinylated ICAM-1/CD54 His&Avi Tag, Human (Cat. No. UA010854) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind ICAM-1/CD54 Recombinant Rabbit mAb (S-906-48) (Cat. No. S0B0795) with EC50 of 3.38-4.90 ng/ml.
