1μg (R: reducing condition, N: non-reducing condition).
Biotinylated CD4/LEU3 His&Avi Tag Protein, Cynomolgus
Biotinylated CD4/LEU3 His&Avi Tag Protein, Cynomolgus
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Product Details
Product Details
Product Specification
| Species | Cynomolgus |
| Synonyms | T4, IMD79, Leu-3, OKT4D, CD4mut |
| Accession | P79185 |
| Amino Acid Sequence | Lys26-Pro396, with C-terminal His and Avitag |
| Expression System | HEK293 |
| Molecular Weight | 50-55kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Biotin |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Gavin R Owen, (2016) Human CD4 Metastability Is a Function of the Allosteric Disulfide Bond in Domain 2. Biochemistry. 55(15):2227-37. |
Background
The cluster of differentiation 4 receptor (CD4) is a 55kDa type I integral membrane glycoprotein which plays a substantial role during adaptive immune responses. In T-cells, CD4 mediates maturation, stabilizes T-cell receptor interactions with peptide-major histocompatibility complex class II (MHCII) complexes on antigen presenting cells, and mediates intracellular T-cell signalling.2 In addition to T-cells, CD4 has also been shown to be expressed on monocytes, monocyte-derived macrophages, Langerhans cells, B lymphocytes, dendritic cells, eosinophils, megakaryocytes and mast cells. The role of CD4 during immune function can also be diverted by the human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein gp120 as the viral receptor utilizes CD4 as the primary receptor as well as the co-receptor CCR5/CXCR4 during the entry process.
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SDS-PAGE
ELISA
Immobilized Biotinylated CD4/LEU3 His&Avi Tag, Cynomolgus (Cat. No. UA010832) at 2 μg/mL on Streptavidin precoated (0.5μg/well) plate, can bind Anti-Human CD4 Monoclonal Antibody(Ibalizumab) with EC50 of 2.21-2.95ng/ml.
