2μg (R: reducing condition, N: non-reducing condition).
Biotinylated CD44/CDw44/ECMR-III Fc&Avi Tag Protein, Human
Biotinylated CD44/CDw44/ECMR-III Fc&Avi Tag Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | CD44,CDW44,CSPG8,ECMR-III,HCELL,HUTCH-I,IN,LHR,MC56,MDU2,MDU3,MIC4,Pgp1,Epican |
| Accession | P16070-1 |
| Amino Acid Sequence | Gln21-Pro220, with C-hIgG1 Fc+Avitag |
| Expression System | HEK293 |
| Molecular Weight | 70-80kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Biotin |
| Tag | Human Fc Tag, Avi Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.4, 5% trehalose |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Review Front Cell Neurosci. 2015 May 7;9:175. eCollection 2015. |
Background
CD44 is a type I transmembrane protein and a member of the cartilage link protein family. Human CD44 has a 20 amino acid (aa) signal sequence, an extracellular domain (ECD) with a 100 aa hyaluronan-binding disulfide-stabilized link region and a 325-530 aa stem region, a 21 aa transmembrane domain, and a 72 aa cytoplasmic domain. CD44 is the major surface hyaluronan (HA) receptor implicated in intercellular and cell-matrix adhesion, cell migration and signaling. CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, hematopoiesis, and tumor metastasis. Transcripts for this gene undergo complex alternative splicing that results in many functionally distinct isoforms.
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SDS-PAGE
ELISA
Immobilized Hyaluronic Acid at 100.0μg/mL (100μL/well) can bind Biotinylated CD44/CDw44/ECMR-III Fc&Avi Tag Protein, Human (Cat. No. UA010909) with EC50 of 2.87-3.80 μg/mL.
