1μg (R: reducing condition, N: non-reducing condition).
B7-2/CD86 His Tag Protein, Mouse
B7-2/CD86 His Tag Protein, Mouse
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$160 USD
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Product Details
Product Details
Product Specification
| Species | Mouse |
| Synonyms | FUN-1，CTLA-4 counter-receptor B7.2，CD28LG2，Activation B7-2 antigen，CD86 Molecule，CD86 Antigen (CD28 Antigen Ligand 2， B7-2 Antigen)，BU63，B70，B-Lymphocyte Activation Antigen B7-2，T-Lymphocyte Activation Antigen CD86，B-Lymphocyte Antigen B7-2，CD86 Antigen，LAB72，B7.2，B7-2 Antigen |
| Accession | Q549Q9 |
| Amino Acid Sequence | Val24-Glu245, with C-terminal His |
| Expression System | HEK293 |
| Molecular Weight | 38-50kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | His Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, 5% trehalose, PH7.4 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1.Greenwald, R.J. et al. (2005) Annu. Rev. Immunol. 23:515. |
Background
CD86, also known as B-lymphocyte activation antigen B7-2 (formerly referred to as B70), is a member of the cell surface immunoglobulin superfamily. Predominantly existing as a monomer on cell surfaces, B7-2 interacts with two co-stimulatory receptors: CD28 and cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4), which are expressed on T cells. This interaction initiates signal pathways that regulate T cell activation and tolerance, cytokine production, and the generation of cytotoxic T lymphocytes (CTLs). B7 family members are transmembrane cell surface molecules that play crucial roles in immune activation and the maintenance of immune tolerance.
B7-2 is highly expressed on activated antigen-presenting cells (APCs), such as B cells, dendritic cells, and monocytes, as well as on vascular endothelial cells. B7-2 and its closely related counterpart, B7-1/CD80, exhibit overlapping yet distinct functional characteristics.
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SDS-PAGE
SEC-HPLC
The purity of B7-2/CD86 His Tag Protein,Mouse is greater than 95% as determined by SEC-HPLC.
ELISA
Immobilized B7-2/CD86 His Tag Protein, Mouse (Cat. No. UA011072) at 2.0μg/mL (100μL/well) can bind CTLA-4/CD152 Fc Chimera Protein, Mouse (Cat. No. UA010773) with EC50 of 46.98-90.08ng/mL.
