Flow cytometric analysis of Human CD18 expression on human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with Allophycocyanin Mouse Anti-Human CD14 Antibody and either Pacific Blue Mouse IgG1, κ Isotype Control (Left panel) or SDT Pacific Blue Mouse Anti-Human CD18 Antibody (Right panel) at 5 μl/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Pacific Blue Mouse Anti-Human CD18 Antibody (S-R487)
Pacific Blue Mouse Anti-Human CD18 Antibody (S-R487)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD18 |
| Synonyms | Integrin beta-2; Cell surface adhesion glycoproteins LFA-1/CR3/p150,95 subunit beta; Complement receptor C3 subunit beta; MFI7; ITGB2 |
| Location | Cell membrane |
| Accession | P05107 |
| Clone Number | S-R487 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | human PBMC |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | Pacific Blue |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Hu |
Background
CD18, also known as integrin β2, is a 95 kDa glycoprotein that is a crucial component of several β2 integrins. These integrins are heterodimeric type I transmembrane proteins composed of a variable alpha chain (CD11a-d) and the common beta chain CD18. The CD18 protein is encoded by the ITGB2 gene in humans. It plays a significant role in cellular adhesion and immune responses. When CD18 binds with different alpha chains, it forms various heterodimers such as lymphocyte function-associated antigen-1 (LFA-1) with CD11a, and Macrophage-1 antigen receptor (Mac-1) with CD11b. These heterodimers are involved in processes like leukocyte adhesion to endothelial cells, phagocytosis, and immune cell activation. Deficiencies in CD18 expression can lead to adhesion defects in white blood cells, impairing the immune system's ability to fight infections.
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