Flow cytometric analysis of Mouse Ki67 expression on proliferating NIH/3T3 cells. Cells from the NIH/3T3 (Mouse embryonic fibroblast) was stained with either Alexa Fluor® 647 Rat IgG2a, κ Isotype Control (Black line histogram) or SDT Alexa Fluor® 647 Rat Anti-Mouse Ki67 Antibody (Red line histogram) at 5 μl/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Alexa Fluor® 647 Rat Anti-Mouse Ki67 Antibody (S-R595)
Alexa Fluor® 647 Rat Anti-Mouse Ki67 Antibody (S-R595)
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$195.00 USD
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ki67 |
| Synonyms | Proliferation marker protein Ki-67; Antigen identified by monoclonal antibody Ki-67 homolog (Antigen KI-67 homolog; Antigen Ki67 homolog); Mki67 |
| Location | Nucleus |
| Accession | E9PVX6 |
| Clone Number | S-R595 |
| Antibody Type | Rat mAb |
| Isotype | IgG2a,k |
| Application | ICFCM |
| Reactivity | Ms |
| Positive Sample | NIH/3T3 |
| Purification | Protein G |
| Concentration | 0.05mg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICFCM | 5μl per million cells in 100μl volume | Ms |
Background
Ki67 protein is a non-histone nuclear protein encoded by the MKI67 gene, widely used as a proliferation marker in pathology. It is expressed during all active phases of the cell cycle—G1, S, G2, and M—but is absent in quiescent (G0) cells. Ki67 has multiple molecular functions, including forming the perichromosomal layer to prevent chromosome aggregation during mitosis and organizing heterochromatin. It also facilitates chromosome attachment to the mitotic spindle and individual chromosome mobility. In addition to its role in cell division, Ki67 is a valuable prognostic and predictive indicator in cancer diagnosis, with its expression levels correlating with tumor cell proliferation rates.
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