Determined by its ability to induced alkaline phosphatase production by C3H10T1/2 Clone8 cells. The EC50 for this effect is less than 5ng/ml.
Wnt-3a Protein, Human
Wnt-3a Protein, Human
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$276 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Antigen | wnt-3a |
| Synonyms | Wnt-3a,wingless-type MMTV integration site family,member 3A,Wnt3a, |
| Accession | P56704 |
| Amino Acid Sequence | Ser19-Lys352 with a fusion tag at the C-terminal |
| Expression System | HEK293 |
| Molecular Weight | 70-72 kDa (Reducing) |
| Purity | >75% by SDS-PAGE |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized powder |
| Storage Buffer |
20 mM citrate sodium, pH6.5.
|
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference |
1.Kyun ML, Kim SO, Lee HG, Hwang JA, Hwang J, Soung NK, Cha-Molstad H, Lee S, Kwon YT, Kim BY, Lee KH. Wnt3a Stimulation Promotes Primary Ciliogenesis through β-Catenin Phosphorylation-Induced Reorganization of Centriolar Satellites. Cell Rep. 2020 Feb 4;30(5):1447-1462. 2.Barrow J.Wnt/planar cell polarity signaling: an important mechanism to coordinate growth and patterning in the limb.Organogenesis. 2011; 7: 260-266 3.Tebroke J, Lieverse JE, Säfholm J, Schulte G,
Nilsson G, Rönnberg E. Wnt-3a Induces Cytokine Release in Human Mast Cells.
Cells. 2019 Nov 1;8(11):1372.
|
Background
Wnt signaling is a key signaling pathway that controls a wide variety of cell fates, such as cell movement, polarity, and differentiation during developmental processes, and regulates the homeostasis of adult tissues. Therefore, abnormal regulation of Wnt signaling results in developmental disorders and various human diseases, including cancer.
The canonical pathway regulates the expression of various genes via β-catenin stabilization and the noncanonical pathway is known to regulate a number of cellular processes, such as cell polarity and fate decision.
The Wnt lipoglycoproteins form a family of 19 secreted ligands that are recognized by 10 Frizzled receptors (FZD1–10), G protein-coupled receptors that associate with various coreceptors. Wnt ligands and their receptors participate in numerous possible ligand/receptor/coreceptor interactions and signal through different downstream pathways, which have been divided into three branches; these branches include the β-catenin-dependent or WNT/β-catenin pathway and an additional network of β-catenin-independent pathways, such as planar cell polarity (PCP)-like signaling pathways and G protein-dependent signaling pathways.
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Bioactivity
SDS-PAGE
2μg (R: reducing conditions, N: non-reducing conditions).
