WB result of VCP Recombinant Rabbit mAb
Primary antibody: VCP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: K562 whole cell lysate 20 µg
Lane 3: MCF7 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 89 kDa
Observed MW: 100 kDa
VCP Recombinant Rabbit mAb (S-1818-121)
VCP Recombinant Rabbit mAb (S-1818-121)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | VCP |
| Synonyms | Transitional endoplasmic reticulum ATPase; TER ATPase; 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein (VCP); HEL-220; HEL-S-70 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P55072 |
| Clone Number | S-1818-121 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, K562, MCF7, SH-SY5Y, NIH/3T3, mouse brain, C6, rat brain |
| Predicted Reactivity | Bv, Pg |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:200 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
VCP (Valosin-Containing Protein) is a highly conserved AAA+ ATPase involved in a wide range of cellular functions, including protein quality control, endoplasmic reticulum-associated degradation (ERAD), autophagy, mitochondrial function regulation, cell cycle control, DNA damage response, and the formation and clearance of stress granules. By interacting with various cofactors, VCP recognizes ubiquitinated proteins and assists in their unfolding and degradation, thereby maintaining cellular protein homeostasis. In the nervous system, VCP plays a critical role in neurodegenerative diseases, where its mutations can lead to protein aggregation and organelle dysfunction, contributing to neuronal pathology. Additionally, VCP is implicated in cardiovascular diseases, with its dysfunction associated with myocardial ischemia/reperfusion injury, cardiac hypertrophy, and heart failure. Given its involvement in multiple diseases, VCP has emerged as a potential therapeutic target, particularly for neurodegenerative and cardiovascular conditions.
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Western Blot
WB result of VCP Recombinant Rabbit mAb
Primary antibody: VCP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 89 kDa
Observed MW: 100 kDa
WB result of VCP Recombinant Rabbit mAb
Primary antibody: VCP Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Lane 2: rat brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 89 kDa
Observed MW: 100 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebral cortex. Anti-VCP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-VCP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-VCP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-VCP antibody was used at 1/200 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in MCF7 cells. Anti-VCP antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
