2μg (R: reducing condition, N: non-reducing condition).
USP47 Protein
USP47 Protein
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$416 USD
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | TRFP |
| Accession | Q96K76 |
| Amino Acid Sequence | Met1-Leu157 MVPGEENQLVPKEDVFWRCRQNIFDEMKKKFLQIENAAEEPRVLCIIQDTTNSKTVNERITLNLPASTPVRKLFEDVANKVGYINGTFDLVWGNGINTADMAPLDHTSDKSLLDANFEPGKKNFLHLTDKDGEQPQILLEDSSAGEDSVHDRFIGPL |
| Expression System | E.coli |
| Molecular Weight | 15kDa |
| Purity | ＞95% by SDS-PAGE&RP-HPLC |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 200mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, -20 to -70 °C as supplied; 6 months, -20 to -70 °C under sterile conditions after reconstitution; 1 week, 2 to 8 °C under sterile conditions after reconstitution; Please avoid repeated freeze-thaw cycles. |
| Reference | 1. Hu Lei, Han-Zhang Xu, Hui-Zhuang Shan, Meng Liu, Ying Lu, Targeting USP47 overcomes tyrosine kinase inhibitor resistance and eradicates leukemia stem/progenitor cells in chronic myelogenous leukemia. Nat Commun. 2021 Jan 4; 12(1):51. |
Background
Ubiquitin-specific peptidase 47 (USP47) is a member of the USP subfamily of DUBs. Similaring to other USPs, USP47 regulates cellular activities by removing ubiquitin conjugates from diverse substrates and, thereby, altering their stabilities, localizations, or activities. Specifically, USP47 deubiquitinates and stabilizes MAPK, DNA polymerase β (Polβ), E-cadherin, β catenin, SNAIL, YAP, β-Trcp, and katanin-p60. Hence, USP47 is involved in cell proliferation, cell survival, DNA damage repair, NLRP3 inflammasome activation, and epithelial-mesenchymal transition. USP47 plays an important role in cancers such as gastric cancer, medulloblastoma, and colorectal cancer.
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SDS-PAGE
RP-HPLC
