WB result of TRIM11 Recombinant Rabbit mAb
Primary antibody: TRIM11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: THP-1 whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: HeLa whole cell lysate 20 µg
Lane 5: Jurkat whole cell lysate 20 µg
Lane 6: HEK-293 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
This blot was developed with high sensitivity substrate
TRIM11 Recombinant Rabbit mAb (S-2913-79)
TRIM11 Recombinant Rabbit mAb (S-2913-79)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TRIM11 |
| Synonyms | E3 ubiquitin-protein ligase TRIM11; Protein BIA1; RING finger protein 92; Tripartite motif-containing protein 11; RNF92 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | Q96F44 |
| Clone Number | S-2913-79 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC |
| Reactivity | Hu, Ms, Rt, Mk |
| Positive Sample | Raji, THP-1, A431, HeLa, Jurkat, HEK-293, NIH/3T3, C6, COS-7 |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Mk |
| ICC | 1:400 | Hu |
Background
TRIM11 is an E3 ubiquitin ligase of the TRIM family that suppresses proteotoxicity by acting as a dual-function chaperone-disaggregase and proteasome activator: it binds misfolded or aggregation-prone proteins such as tau, α-synuclein and Atxn1-82Q, prevents their aggregation, dissolves pre-formed fibrils, and then targets them for SUMOylation and proteasomal degradation by displacing the de-ubiquitinase USP14 from the 26S proteasome, thereby enhancing overall proteolysis; concurrently TRIM11 ubiquitinates and destabilizes specific regulatory proteins (Axin1, PAX6, ARC105, PHOX2B, TBK1, PHLPP1) to modulate Wnt/β-catenin, neurogenesis, TGF-β, IFN-β and AKT signaling, and its expression is up-regulated in many cancers where it drives proliferation, invasion, glycolysis and ferroptosis resistance, while its down-regulation in Alzheimer’s disease contributes to toxic tau accumulation, positioning TRIM11 as a central node linking protein-quality-control, innate immunity and oncogenesis.
Picture
Picture
Western Blot
WB result of TRIM11 Recombinant Rabbit mAb
Primary antibody: TRIM11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
This blot was developed with high sensitivity substrate
WB result of TRIM11 Recombinant Rabbit mAb
Primary antibody: TRIM11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
This blot was developed with high sensitivity substrate
WB result of TRIM11 Recombinant Rabbit mAb
Primary antibody: TRIM11 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: COS-7 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 53 kDa
Observed MW: 53 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC shows positive staining in Raji cells. Anti-TRIM11 antibody was used at 1/400 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
