WB result of TOP2A Recombinant Rabbit mAb
Primary antibody: TOP2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: WI-38 whole cell lysate 20 µg
Lane 2: SK-BR-3 whole cell lysate 20 µg
Lane 3: A549 whole cell lysate 20 µg
Lane 4: A431 whole cell lysate 20 µg
Lane 5: HeLa whole cell lysate 20 µg
Low expression control: WI-38 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 174 kDa
Observed MW: 190 kDa
TOP2A Recombinant Rabbit mAb (S-513-107)
TOP2A Recombinant Rabbit mAb (S-513-107)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TOP2A |
| Synonyms | DNA topoisomerase 2-alpha; TOP2 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus |
| Accession | P11388 |
| Clone Number | S-513-107 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | SK-BR-3, A549, A431, HeLa, Neuro-2a, mouse testis |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
TOP2A (DNA topoisomerase IIα) is a crucial nuclear enzyme highly expressed during the S and G2/M phases of the cell cycle, responsible for resolving topological challenges arising from DNA replication and chromosome segregation; it functions by creating transient double-strand breaks in DNA, allowing another DNA duplex to pass through the gap before resealing the ends, thereby effectively relieving supercoiling tension, decatenating interlinked DNA rings, and ensuring the accurate separation of sister chromatids during anaphase. As a hallmark of cell proliferation, TOP2A exhibits high activity in rapidly dividing tissues, and its dysfunction can lead to genomic instability and chromosomal aberrations; furthermore, it serves as a primary target for important anticancer drugs such as anthracyclines (e.g., doxorubicin) and epipodophyllotoxins (e.g., etoposide), which exert their cytotoxic effects by stabilizing the TOP2A-DNA covalent complex to induce lethal double-strand breaks, ultimately inhibiting tumor cell growth.
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Western Blot
WB result of TOP2A Recombinant Rabbit mAb
Primary antibody: TOP2A Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Neuro-2a whole cell lysate 20 µg
Lane 2: mouse testis lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 174 kDa
Observed MW: 190 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human testis. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human tonsil. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse colon. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat testis. Anti-TOP2A antibody was used at 1/2000 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-TOP2A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
