WB result of Tau (phospho S404) Recombinant Rabbit mAb
Primary antibody: Tau (phospho S404) Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: old mouse brain lysate 20 µg
Lane 2: phosphatase treated old mouse brain lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 46 kDa
Observed MW: 48 kDa
Tau (phospho S404) Recombinant Rabbit mAb (S-982-20)
Tau (phospho S404) Recombinant Rabbit mAb (S-982-20)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | Microtubule-associated protein tau, Neurofibrillary tangle protein, Paired helical filament-tau (PHF-tau), MAPT, MAPTL, MTBT1 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | P10636-8 |
| Clone Number | S-982-20 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Ms |
| Predicted Reactivity | Cz, Gor, Pr, Or, Hu, Rt, Mq, Bv, Sq, Gt, Bb |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 |
Background
Tau protein is a microtubule-associated protein (MAP) that is abundantly expressed in neurons of the central nervous system and is scarcely found in other cell types. It primarily contributes to maintaining the structure and stability of microtubules, which are crucial for the normal function and axonal transport of neurons. Phosphorylation is an important mode of regulating Tau protein function. Abnormal levels or functions of Tau (phospho S404) may be associated with specific types of neurodegenerative diseases.
Picture
Picture
Western Blot
Dot Blot
Dot blot result of Tau (phospho S404) Recombinant Rabbit mAb
Lane 1: Tau (phospho S404) peptide
Lane 2: Tau non-phospho peptide (around S404)
Primary antibody: Tau (phospho S404) Recombinant Rabbit mAb at 1/1000 dilution
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
This blot was developed with high sensitivity substrate
