WB result of TAGLN/Transgelin Recombinant Rabbit mAb
Primary antibody: TAGLN/Transgelin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 23 kDa
TAGLN/Transgelin Recombinant Rabbit mAb (S-1715-59)
TAGLN/Transgelin Recombinant Rabbit mAb (S-1715-59)
Price:
Regular price
$130.00 SGD
Regular price
Sale price
$130.00 SGD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | TAGLN/Transgelin |
| Synonyms | 22 kDa actin-binding protein; Protein WS3-10; Smooth muscle protein 22-alpha (SM22-alpha); SM22; WS3-10 |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | Q01995 |
| Clone Number | S-1715-59 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | HeLa, Caco-2, mouse bladder, rat bladder |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:2000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu, Ms, Rt |
Background
The TAGLN protein, also known as transgelin, is a member of the calponin family of actin-binding proteins. It is highly expressed in vascular and visceral smooth muscle cells and serves as an early marker of smooth muscle differentiation. TAGLN is involved in calcium-independent smooth muscle contraction and acts as a tumor suppressor. Its expression is often lost during cell transformation and tumor development, coinciding with cellular plasticity. In addition to its role in smooth muscle, TAGLN has been implicated in various cellular processes, including epithelial cell differentiation and immune responses. The protein consists of an N-terminal calponin homology (CH) domain and a C-terminal calponin-like (CLIK) domain. Studies have shown that TAGLN can inhibit the proliferation and invasion of cancer cells, such as colorectal carcinoma cells, and promote apoptosis. Furthermore, TAGLN expression is upregulated in certain types of tumors, such as NF1-associated malignant peripheral nerve sheath tumors, due to hypomethylation in its promoter region. Overall, TAGLN plays a multifaceted role in cellular function and disease, particularly in the context of smooth muscle and cancer biology.
Picture
Picture
Western Blot
WB result of TAGLN/Transgelin Recombinant Rabbit mAb
Primary antibody: TAGLN/Transgelin Recombinant Rabbit mAb at 1/2000 dilution
Lane 1: mouse skeletal muscle lysate 10 µg
Lane 2: mouse bladder lysate 10 µg
Negative control: mouse skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 23 kDa
WB result of TAGLN/Transgelin Recombinant Rabbit mAb
Primary antibody: TAGLN/Transgelin Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat skeletal muscle lysate 20 µg
Lane 2: rat bladder lysate 20 µg
Negative control: rat skeletal muscle lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 23 kDa
Observed MW: 23 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon. Anti-TAGLN/Transgelin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cardiac muscle. Anti-TAGLN/Transgelin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human prostate. Anti-TAGLN/Transgelin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human skeletal muscle. Anti-TAGLN/Transgelin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-TAGLN/Transgelin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-TAGLN/Transgelin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-TAGLN/Transgelin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cardiac muscle. Anti-TAGLN/Transgelin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat stomach. Anti-TAGLN/Transgelin antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
