WB result of STK39 Recombinant Rabbit mAb
Primary antibody: STK39 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: Jurkat whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: MDA-MB-231 whole cell lysate 20 µg
Lane 4: SH-SY5Y whole cell lysate 20 µg
Lane 5: Raji whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 65 kDa
This blot was developed with high sensitivity substrate
STK39 Recombinant Rabbit mAb (S-3630-15)
STK39 Recombinant Rabbit mAb (S-3630-15)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | STK39 |
| Synonyms | STE20/SPS1-related proline-alanine-rich protein kinase; DCHT; Serine/threonine-protein kinase 39; PASK; SPAK |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Nucleus |
| Accession | Q9UEW8 |
| Clone Number | S-3630-15 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | Jurkat, MCF7, MDA-MB-231, SH-SY5Y, Raji, HepG2, mouse brain, rat brain |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
STK39 (Serine/Threonine Kinase 39), also known as SIK3 or salt-inducible kinase 3, is a multifunctional enzyme belonging to the AMP-activated protein kinase (AMPK) family within the broader serine/threonine kinase group, playing pivotal roles in regulating cellular metabolism, ion transport, and circadian rhythms. Primarily activated by upstream kinases such as LKB1, STK39 functions as a critical mediator in response to hormonal signals like aldosterone and insulin, where it phosphorylates specific substrates including the sodium-potassium-chloride cotransporter NKCC1 and various transcriptional co-repressors (such as HDAC4 and CRTC2) to modulate electrolyte balance, glucose homeostasis, and lipid metabolism. Beyond its metabolic duties, STK39 is integral to the molecular clock mechanism by influencing the periodicity of circadian gene expression through the regulation of CREB signaling pathways, and emerging research highlights its involvement in pathological conditions, including its potential tumor-suppressive or oncogenic roles depending on tissue context, as well as its contribution to neurodevelopmental disorders and pain sensitivity via neuronal signaling modulation.
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Western Blot
WB result of STK39 Recombinant Rabbit mAb
Primary antibody: STK39 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 65 kDa
WB result of STK39 Recombinant Rabbit mAb
Primary antibody: STK39 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse liver lysate 20 µg
Lane 2: mouse brain lysate 20 µg
Low expression control: mouse liver lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 70 kDa
This blot was developed with high sensitivity substrate
WB result of STK39 Recombinant Rabbit mAb
Primary antibody: STK39 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Lane 2: rat brain lysate 20 µg
Low expression control: rat liver lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 59 kDa
Observed MW: 65, 70 kDa
This blot was developed with high sensitivity substrate
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human cerebellum. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human pancreatic cancer. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse cerebral cortex. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat cerebral cortex. Anti-STK39 antibody was used at 1/500 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HepG2 cells. Anti-STK39 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
