SIRP-α Fc Chimera Protein, Human
SIRP-α Fc Chimera Protein, Human
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | SIRP-α Fc Chimera protein ;BIT, BITbrain-immunoglobulin-like molecule with tyrosine-based activation motifs, Brain Ig-like molecule with tyrosine-based activation motifs, CD172 antigen-like family member A, CD172a antigen, CD172a, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MFR, MFRtyrosine phosphatase SHP substrate 1, MyD-1 antigen, MYD1, MYD-1, P84, protein tyrosine phosphatase, non-receptor type substrate 1, PTPNS1, SHP substrate 1, SHPS1, SHPS-1, SHPS1CD172A, signal-regulatory protein alpha, Signal-regulatory protein alpha-1, Signal-regulatory protein alpha-2, Signal-regulatory protein alpha-3, SIRP alpha, SIRPA, SIRPalpha, Sirp-alpha-1, SIRPalpha2, Sirp-alpha-2, Sirp-alpha-3, SIRPtyrosine-protein phosphatase non-receptor type substrate 1 |
| Accession | P78324 |
| Amino Acid Sequence | Glu31-Arg370, with C-terminal Human IgG1 Fc |
| Expression System | HEK293 |
| Molecular Weight | 70~80 kDa(Reducing) |
| Purity | ＞95%, by SDS-PAGE under reducing conditions |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human Fc Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS, pH7.2, 5% trehalose |
| Reconstitution | Reconstitute at less than 1 mg/mL according to the size in ultrapure water after rapid centrifugation . |
| Stability & Storage |
· 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
Background
Protein tyrosine phosphatases (PTPases) SHP-1 and SHP-2 are critical regulators in the intracellular signaling pathways that result in cell responses such as mitosis, differentiation, migration, survival, transformation or death. SHP-2 is a signal transducer for several receptor tyrosine kinases and cytokine receptors. A novel SHP-2 associated glycoprotein was recently cloned from human, rat, mouse and cattle by several labs and was designated SIRPalpha, SHPS-1, MyD-1, BIT and p84. SIRPalpha is a new gene family containing at least fifteen members. SIRPalpha is a substrate of many activated tyrosine kinases such as insulin receptor, EGFR, PDGFR and src, and a specific docking protein for SHP-2. SIRPalpha has regulatory effects on cellular responses induced by serum, growth factors, insulin, oncogenes, growth hormones and cell adhesion and plays a general role in different physiological and pathological processes.
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Bioactivity
Immobilized SIRP-α Fc Chimera, Human (Cat. No. UA010005) at 2.0μg/mL (100μL/well) can bind Biotinylated CD47 Fc&Avi Tag, Human (Cat. No. UA010444) with EC50 of 45.92-64.38 ng/mL.
SDS-PAGE
ELISA
Immobilized SIRP-α Fc Chimera Protein, Human (Cat. No. UA010014) at 2.0μg/mL (100μL/well) can bind CD47 His Tag Protein, Human (Cat. No. UA010014) with EC50 of 0.77-0.97ng/mL.
