WB result of SHMT2/SHMT Recombinant Rabbit mAb
Primary antibody: SHMT2/SHMT Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: PC-3 whole cell lysate 20 µg
Lane 2: MCF7 whole cell lysate 20 µg
Lane 3: HeLa whole cell lysate 20 µg
Lane 4: HepG2 whole cell lysate 20 µg
Lane 5: OVCAR-3 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 50 kDa
SHMT2/SHMT Recombinant Rabbit mAb (S-1581-5)
SHMT2/SHMT Recombinant Rabbit mAb (S-1581-5)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | SHMT2/SHMT |
| Synonyms | Serine hydroxymethyltransferase, mitochondrial; Glycine hydroxymethyltransferase; Serine methylase |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm, Nucleus, Mitochondrion |
| Accession | P34897 |
| Clone Number | S-1581-5 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | PC-3, MCF7, HeLa, HepG2, OVCAR-3, mouse liver, rat liver |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:1000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu |
Background
SHMT2 protein, or serine hydroxymethyltransferase 2, is a mitochondrial enzyme that plays a pivotal role in one-carbon metabolism by catalyzing the reversible reaction of serine and tetrahydrofolate to glycine and 5,10-methylene tetrahydrofolate. This reaction is crucial for the synthesis of glycine and the provision of one-carbon units necessary for DNA synthesis, particularly through the production of thymidine and pyrimidine nucleotides. SHMT2 is expressed ubiquitously, with particularly high levels in the liver, and its activity is suggested to be the primary source of intracellular glycine. It is also associated with maintaining normal cellular methylation patterns, DNA stability, and genetic mutations. Abnormal expression of SHMT2 has been linked to various cancers, including colorectal, renal cell, head and neck, hepatocellular carcinoma, non-small cell lung cancer, breast cancer, and prostate cancer, where it contributes to cancer cell proliferation and survival by supporting metabolic reprogramming. Therefore, SHMT2 is not only a key player in cellular metabolism but also a potential therapeutic target in cancer treatment.
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Western Blot
WB result of SHMT2/SHMT Recombinant Rabbit mAb
Primary antibody: SHMT2/SHMT Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: mouse liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 50 kDa
WB result of SHMT2/SHMT Recombinant Rabbit mAb
Primary antibody: SHMT2/SHMT Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: rat liver lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 56 kDa
Observed MW: 50 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human endometrial cancer. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human gastric cancer. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse stomach. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded rat kidney. Anti-SHMT2/SHMT antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-SHMT2/SHMT antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
