WB result of sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb
Primary antibody: sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HUVEC whole cell lysate 20 µg
Lane 2: A431 whole cell lysate 20 µg
Lane 3: Jurkat whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 180 kDa
sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb (S-807-137)
sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb (S-807-137)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | sFlt-1/VEGF Receptor 1 |
| Synonyms | VEGFR-1, FRT |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P17948 |
| Clone Number | S-807-137 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu, Ms |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 |
Background
Vascular endothelial growth factor (VEGF) represents a growth factor with important pro-angiogenic activity, having a mitogenic and an anti-apoptotic effect on endothelial cells, increasing the vascular permeability, promoting cell migration, etc. VEGF binds to tyrosine kinase cell receptors (VEGFRs): VEGFR-1 [Fms-like tyrosine kinase 1 (Flt-1)], VEGFR-2 [kinase insert domain receptor (KDR) in human; fetal liver kinase 1 (Flk-1) in mouse] and VEGFR-3 [Fms-like tyrosine kinase 4 (Flt-4)]. While VEGFR-1 and VEGFR-2 are expressed predominantly on vascular endothelial cells, VEGFR-3 is expressed especially on lymphatic endothelial cells.
Picture
Picture
Western Blot
WB result of sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb
Primary antibody: sFlt-1/VEGF Receptor 1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: mouse brain whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 150 kDa
Observed MW: 180 kDa
IP
sFlt-1/VEGF Receptor 1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating sFlt-1/VEGF Receptor 1 in 0.4 mg A431 whole cell lysate.
Western blot was performed on the immunoprecipitate using sFlt-1/VEGF Receptor 1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: A431 whole cell lysate 10 µg (Input)
Lane 2: sFlt-1/VEGF Receptor 1 Rabbit mAb IP in A431 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in A431 whole cell lysate
Predicted MW: 150 kDa
Observed MW: 180 kDa
