WB result of mCherry Recombinant Rabbit mAb
Primary antibody: mCherry Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: 293T transfected with empty vector whole cell Lysate 20 µg
Lane 3: 293T transfected with Histone H3-mCherry-E-tag whole cell Lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 48 kDa
mCherry Recombinant Rabbit mAb (S-1049-67)
mCherry Recombinant Rabbit mAb (S-1049-67)
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$100.00 USD
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$100.00 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | mCherry fluorescent protein |
| Immunogen | Recombinant Protein |
| Accession | X5DSL3 |
| Clone Number | S-1049-67 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB |
| Reactivity | Species Independent |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied. |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | null |
Background
mCherry is a widely used red fluorescent protein in biotechnology, exhibiting several key properties and applications. mCherry originates from the coral Discosoma species and serves as a monomeric red fluorescent protein. It absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm. Unlike GFP (Green Fluorescent Protein) and its variants derived from jellyfish, mCherry possesses distinct red fluorescent properties. It is commonly used as a red fluorescent tag protein for labeling and localizing molecular components within cells.
Picture
Picture
Western Blot
