WB result of mCherry Recombinant Rabbit mAb
Primary antibody: mCherry Recombinant Rabbit mAb at 1/5000 dilution
Lane 1: 293T whole cell lysate 20 µg
Lane 2: 293T transfected with empty vector whole cell Lysate 20 µg
Lane 3: 293T transfected with Histone H3-mCherry-E-tag whole cell Lysate 5 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 27 kDa
Observed MW: 48 kDa
mCherry Recombinant Rabbit mAb (S-1049-67)
mCherry Recombinant Rabbit mAb (S-1049-67)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Synonyms | mCherry fluorescent protein |
| Immunogen | Recombinant Protein |
| Accession | X5DSL3 |
| Clone Number | S-1049-67 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ChIP |
| Reactivity | Species Independent |
| Cross Reactivity | This antibody is reactive against variants of RFP such asmRFP, mPlum, mOrange, PA-mCherry and mScarlet |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | |
| ChIP | 1:20-1:50 |
Background
mCherry is a widely used red fluorescent protein in biotechnology, exhibiting several key properties and applications. mCherry originates from the coral Discosoma species and serves as a monomeric red fluorescent protein. It absorbs light in the range of 540-590 nm and emits light in the range of 550-650 nm. Unlike GFP (Green Fluorescent Protein) and its variants derived from jellyfish, mCherry possesses distinct red fluorescent properties. It is commonly used as a red fluorescent tag protein for labeling and localizing molecular components within cells.
Picture
Picture
Western Blot
ChIP
Chromatin immunoprecipitation (ChIP) was performed on 293F cells were either untransfected (left panel) or transfected with a mCherry-tagged human H3 construct (right panel) cross - linked with 1% formaldehyde for 10 min, then chromatin was fragmented by sonication. Parallel reactions used mCherry Recombinant Rabbit mAb (S-1049-67) and Rabbit mAb IgG Isotype Control (SDT-R173) at 1:50 for immunoprecipitation.
Post-immunoprecipitation, both samples were washed, eluted, and cross-links reversed. Purified DNA was analyzed by qPCR.
qPCR showed the enrichment of RPL30, AFM and SAT-α
in mCherry Recombinant Rabbit mAb (S-1049-67)-
immunoprecipitated sample.
