Measured in a cell proliferation assay using HT-29 human colon adenocarcinoma cells), the EC50 for this effect is less than 10ng/ml.
LIGHT/TNFSF14 Protein, Human
LIGHT/TNFSF14 Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand (HVEM-L; Herpesvirus entry mediator ligand); |
| Accession | O43557 |
| Amino Acid Sequence | Asp74-Val240, with N-terminal hIgG1 Fc |
| Expression System | HEK293 |
| Molecular Weight | 50 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Huamn IgG1 Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 50mM Glycine, 300mM NaCl, pH9.5. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | J Clin Invest. 2001 Dec;108(12):1771-80. |
Background
TNFSF14, also known as tumor necrosis factor ligand superfamily member 14 or LIGHT (homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), is a cytokine belonging to the tumor necrosis factor (TNF) superfamily. TNFSF14 is a type II transmembrane protein that can be cleaved to produce a soluble form. It functions as a ligand for two receptors, herpes virus entry mediator (HVEM) and lymphotoxin beta receptor (LTβR), and is involved in regulating immune responses, inflammation, and lymphoid organ development. TNFSF14 has been implicated in various physiological and pathological processes, including the modulation of T cell activation, the regulation of immune cell migration, and the promotion of antitumor immunity.
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Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
ELISA
Immobilized LIGHT/TNFSF14 Protein, Human (Cat. No. UA040352) at 5.0μg/mL (100μL/well) can bind LTBR/TNFRSF3 His tag Protein, Human (Cat. No. UA010534) with EC50 of 0.25-0.31ug/mL.
Immobilized LIGHT/TNFSF14 Protein, Human (Cat. No. UA040352) at 5/mL (100μL/well) can bind LTBR/TNFRSF3 His tag Protein, Human (Cat. No. UA010534) with EC50 of 0.25-0.31 μg/mL.
