Measured in a cell cytotoxicity assay using HT-29 human colon adenocarcinoma cells, the IC50 for this effect is 0.58-1.7 ng/ml.
LIGHT/TNFSF14 Protein, Human
LIGHT/TNFSF14 Protein, Human
Price:
Regular price
$120 USD
Regular price
Sale price
$120 USD
Unit price
per
For shipping services or bulk orders, you may request a quotation.
Secure checkout with
View full details
Product Details
Product Details
Product Specification
| Species | Human |
| Synonyms | Tumor necrosis factor ligand superfamily member 14; Herpes virus entry mediator ligand (HVEM-L; Herpesvirus entry mediator ligand); |
| Accession | O43557 |
| Amino Acid Sequence | Asp74-Val240, with N-terminal hIgG1 Fc |
| Expression System | HEK293 |
| Molecular Weight | 50 kDa (Reducing) |
| Purity | >95% by SDS-PAGE and HPLC. |
| Endotoxin | <0.1EU/μg |
| Conjugation | Unconjugated |
| Tag | Human IgG1 Fc |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 50mM Glycine, 300mM NaCl, pH9.5. |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. |
| Reference | J Clin Invest. 2001 Dec;108(12):1771-80. |
Background
TNFSF14, also known as tumor necrosis factor ligand superfamily member 14 or LIGHT (homologous to lymphotoxins, exhibits inducible expression, and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes), is a cytokine belonging to the tumor necrosis factor (TNF) superfamily. TNFSF14 is a type II transmembrane protein that can be cleaved to produce a soluble form. It functions as a ligand for two receptors, herpes virus entry mediator (HVEM) and lymphotoxin beta receptor (LTβR), and is involved in regulating immune responses, inflammation, and lymphoid organ development. TNFSF14 has been implicated in various physiological and pathological processes, including the modulation of T cell activation, the regulation of immune cell migration, and the promotion of antitumor immunity.
Picture
Picture
Bioactivity
SDS-PAGE
2μg (R: reducing condition, N: non-reducing condition).
RP-HPLC
>95% as determined by RP-HPLC.
ELISA
Immobilized Biotinylated Human LTBR, His,Avitag at 1 μg/mL (100 μL/well) on streptavidin precoated plate can bind LIGHT/TNFSF14 Protein, Human (Cat. No. UA040352) with a linear range of 1.1-1.4ng/mL.
