WB result of CDKN2A/p16 INK4A Recombinant Rabbit mAb
Primary antibody: CDKN2A/p16 INK4A Recombinant Rabbit mAb at 1/250 dilution
Lane 1: MCF7 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: HEK-293 whole cell lysate 20 µg
Lane 4: Saos-2 whole cell lysate 20 µg
Negative control: MCF7 whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 16 kDa
Observed MW: 16 kDa
CDKN2A/p16 INK4A Recombinant Mouse mAb (SDT-4677)
CDKN2A/p16 INK4A Recombinant Mouse mAb (SDT-4677)
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$100 USD
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CDKN2A/p16 INK4A |
| Synonyms | Tumor suppressor ARF; Alternative reading frame (ARF); Cyclin-dependent kinase inhibitor 2AImported; p14ARF; CDKN2; MLM |
| Location | Nucleus, Mitochondrion |
| Accession | Q8N726 |
| Clone Number | SDT-4677 |
| Antibody Type | Mouse mAb |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | HeLa, HEK-293, Saos-2 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:250-1:500 | Hu |
| IHC-P | 1:1000 | Hu |
| ICC | 1:500 | Hu |
Background
CDKN2A/p16INK4A is a critical tumor suppressor protein encoded by the CDKN2A gene that functions as a key negative regulator of the cell cycle by specifically inhibiting cyclin-dependent kinases 4 and 6 (CDK4/6), thereby preventing the phosphorylation of the retinoblastoma protein (Rb) and arresting cell progression from the G1 to the S phase to curb uncontrolled proliferation. Often referred to as p16, this protein acts as a vital checkpoint against oncogenic transformation, and its loss of function through genetic deletion, mutation, or epigenetic silencing is a hallmark event in numerous human cancers, including melanoma, pancreatic ductal adenocarcinoma, and glioblastoma; conversely, its overexpression is frequently observed in cells undergoing senescence or in HPV-associated malignancies where it serves as a reliable diagnostic biomarker due to the disruption of the Rb pathway by viral oncoproteins, making p16INK4A not only a fundamental component of cellular defense mechanisms but also a significant target for therapeutic strategies and prognostic assessments in oncology.
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Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human colon cancer. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human colon. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical squamous cell carcinoma. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human cervix. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human bladder cancer. Anti-CDKN2A/p16 INK4A antibody was used at 1/1000 dilution, followed by a HRP Polymer for Mouse IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in HeLa cells (top panel) and negative staining in MCF7 cells (below panel). Anti-CDKN2A/p16 INK4A antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Mouse IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
