WB result of BRG1 Recombinant Rabbit mAb
Primary antibody: BRG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: A549 whole cell lysate 20 µg
Lane 2: HeLa whole cell lysate 20 µg
Lane 3: K562 whole cell lysate 20 µg
Lane 4: NCCIT whole cell lysate 20 µg
Negative control: A549 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 184 kDa
Observed MW: 220 kDa
BRG1 Recombinant Rabbit mAb (S-1513-4)
BRG1 Recombinant Rabbit mAb (S-1513-4)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | BRG1 |
| Synonyms | Transcription activator BRG1, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A (BAF190A), Mitotic growth and transcription activator, Protein BRG-1, Protein brahma homolog 1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, SMARCA4, BAF190A, SNF2B, SNF2L4 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P51532 |
| Clone Number | S-1513-4 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, ICC, ICFCM, IP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Bv |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IP | 1:50 | |
| ICC | 1:500 | |
| ICFCM | 1:500 |
Background
BRG1, also known as SMARCA4, is a protein that serves as the essential ATPase subunit of the SWI/SNF chromatin-remodeling complex. This complex plays a crucial role in regulating gene expression by altering the structure of chromatin, which in turn affects the accessibility of DNA to the transcription machinery. BRG1 functions by maintaining chromatin accessibility at gene transcription start sites (TSSs) and enhancers, which is vital for the binding of transcriptional coactivators like p300 and the subsequent acetylation of histones, such as H3K27. BRG1 has also been implicated in cancer development. In B-cell acute lymphoblastic leukemia (B-ALL), BRG1 overexpression is associated with worse outcomes and promotes the proliferation and survival of leukemia cells. The inhibition of BRG1 leads to cell cycle arrest and increased apoptosis in B-ALL cells, suggesting that BRG1 may be a potential therapeutic target for this type of cancer.
Picture
Picture
Western Blot
WB result of BRG1 Recombinant Rabbit mAb
Primary antibody: BRG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: NIH/3T3 whole cell lysate 20 µg
Lane 2: RAW264.7 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 184 kDa
Observed MW: 220 kDa
WB result of BRG1 Recombinant Rabbit mAb
Primary antibody: BRG1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: C6 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 184 kDa
Observed MW: 220 kDa
FC
Flow cytometric analysis of 4% PFA fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) labelling BRG1 antibody at 1/500 dilution (0.1 μg) / (Red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat Anti - Rabbit IgG Alexa Fluor® 488 was used as the secondary antibody.
IP
BRG1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating BRG1 in 0.4 mg K562 whole cell lysate.
Western blot was performed on the immunoprecipitate using BRG1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/1000 dilution.
Lane 1: K562 whole cell lysate 5 µg (Input)
Lane 2: BRG1 Rabbit mAb IP in K562 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in K562 whole cell lysate
Predicted MW: 184 kDa
Observed MW: 220 kDa
This blot was developed with high sensitivity substrate
Immunocytochemistry
ICC shows positive staining in HeLa cells. Anti-BRG1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
