Flow cytometric analysis of Mouse CD3 expression on C57BL/6 mouse splenocytes. C57BL/6 mouse splenocytes were stained with Alexa Fluor® 647 Rat Anti-Mouse CD19 antibody and SDT Biotin Rat Anti-Mouse CD3 Antibody at 5 μl/test followed by Sav-PE. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Rat Anti-Mouse CD3 Antibody (17A2)
Biotin Rat Anti-Mouse CD3 Antibody (17A2)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | CD3 |
| Synonyms | T-cell surface glycoprotein CD3 zeta chain; T-cell receptor T3 zeta chain; CD247; Cd3z; Tcrz |
| Location | Cell membrane |
| Accession | P24161 |
| Clone Number | 17A2 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse splenocytes |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5μl per million cells in 100μl volume | Ms |
Background
The CD3 protein is a T-cell marker, a complex of four structurally distinct membrane glycoprotein isoforms, 20-50 kDa, comprising extracellular, transmembrane and intracellular domains. CD3 is associated with an heterodimer creating the T-cell receptor (TCR). The CD3 complex is responsible for mediating signal transduction to the internal environment upon antigenic recognition by TCR, causing T-cell proliferation and release of cytokines.
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