Flow cytometric analysis of Human CD16 expression on human peripheral blood. Human peripheral blood was stained with biotin Isotype Control (Left panel) or SDT APC Mouse Anti-Human CD11a antibody (Right panel) at 1.25μl/test followed by Sav-iFluor 488. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
Biotin Mouse Anti-Human CD16 Antibody (S-R440)
Biotin Mouse Anti-Human CD16 Antibody (S-R440)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD16 |
| Synonyms | Low affinity immunoglobulin gamma Fc region receptor III-A; IgG Fc receptor III-A; CD16-II; CD16a antigen; Fc-gamma RIII-alpha (Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcgammaRIIIA); FcR-10; IgG Fc receptor III-2; CD16A; FCGR3A |
| Location | Secreted, Cell membrane |
| Accession | P08637 |
| Clone Number | S-R440 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.2 mg/ml |
| Conjugation | Biotin |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1.25μl per million cells in 100μl volume | Hu |
Background
CD16, also known as FcγRIII, is a low-affinity IgG receptor expressed on various immune cells, including natural killer (NK) cells, monocytes, macrophages, and neutrophils. As a member of the immunoglobulin superfamily, CD16 plays a crucial role in immune responses by binding to the Fc portion of IgG antibodies. This interaction triggers several important immune functions such as phagocytosis, respiratory burst, and antibody-dependent cellular cytotoxicity (ADCC), which are vital for defending against pathogens and cancer cells. CD16 exists in two forms, CD16a and CD16b, with CD16a being predominantly found on NK cells and monocytes, while CD16b is primarily expressed on neutrophils. Its ability to activate NK cells and enhance their cytotoxicity against target cells makes CD16 a significant target in cancer immunotherapy and antibody drug development.
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