The Biotin-KRAS[G12A] activity was detected using HTRF technology. The reaction was performed by incubating the Biotin-KRAS[G12A] protein, GTP and SOS1 at 25℃ for 60 min, adding cRAF at 25℃ for 15 min and adding the beads and keeping the mixture at 25℃ for 60 min, then reading ratio 665/620 with BMG.
Biotin Kras[G12A] Protein, Human
Biotin Kras[G12A] Protein, Human
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Product Details
Product Details
Product Specification
| Species | Human |
| Accession | P01116-2 |
| Amino Acid Sequence | M1-K169 |
| Expression System | E.coli |
| Molecular Weight | 23.5 kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | Avi Tag, His Tag |
| Physical Appearance | Liquid |
| Storage Buffer | 50 mM Tris, 150 mM NaCl, 1 mM DTT, pH7.5 |
| Stability & Storage | Stable for 12 months upon stored at -80℃ from the date of receipt. And avoid repeated freeze-thaws cycles. |
Picture
Picture
Bioactivity
SDS-PAGE
M MW Standard
1 1 μg Biotin-Kras[G12A]
2 3 μg Biotin-Kras[G12A]
