WB result of Bim Recombinant Rabbit mAb
Primary antibody: Bim Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: Raji whole cell lysate 20 µg
Lane 2: Jurkat whole cell lysate 20 µg
Lane 3: A431 whole cell lysate 20 µg
Lane 4: Molt-4 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12, 15, 23 kDa
Observed MW: 12, 15, 23 kDa
Bim Recombinant Rabbit mAb (S-994-29)
Bim Recombinant Rabbit mAb (S-994-29)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Bim |
| Synonyms | Bcl-2-like protein 11, Bcl2-L-11, Bcl2-interacting mediator of cell death, BCL2L11 |
| Immunogen | Synthetic Peptide |
| Accession | O43521 |
| Clone Number | S-994-29 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:4000 | |
| IHC-P | 1:100 |
Background
BIM belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BIM contains a Bcl-2 homology domain 3 (BH3). It has been shown to interact with other members of the BCL-2 protein family, including BCL2, BCL2L1/BCL-X(L), and MCL1, and to act as an apoptotic activator. Bim expression and activity are regulated at the transcriptional, translational and post-translational levels; coordinated expression and activity of Bim shape immune responses, and ensure tissue integrity. Cancer cells develop mechanisms that suppress Bim expression, which allows for tumor progression and metastasis.
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Western Blot
WB result of Bim Recombinant Rabbit mAb
Primary antibody: Bim Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: RAW264.7 whole cell lysate 20 µg
Lane 2: mouse spleen lysate 20 µg
Lane 3: mouse thymus lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12, 15, 23 kDa
Observed MW: 12, 15, 23 kDa
WB result of Bim Recombinant Rabbit mAb
Primary antibody: Bim Recombinant Rabbit mAb at 1/4000 dilution
Lane 1: PC-12 whole cell lysate 20 µg
Lane 2: rat spleen lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 12, 15, 23 kDa
Observed MW: 12, 15, 23 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human breast cancer. Anti-Bim antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervical cancer. Anti-Bim antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human ovarian cancer. Anti-Bim antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung cancer. Anti-Bim antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
