WB result of ATG12 Recombinant Rabbit mAb
Primary antibody: ATG12 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Lane 2: HCT 116whole cell lysate 20 µg
Lane 3: PANC-1 whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 15 kDa
Observed MW: 20, 56 kDa
ATG12 Recombinant Rabbit mAb (S-1143-209)
ATG12 Recombinant Rabbit mAb (S-1143-209)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ATG12 |
| Synonyms | Ubiquitin-like protein ATG12; Autophagy-related protein 12 (APG12-like); APG12; APG12L |
| Immunogen | Synthetic Peptide |
| Location | Cytoplasm |
| Accession | O94817 |
| Clone Number | S-1143-209 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms |
| Positive Sample | HeLa, HCT 116, PANC-1 |
| Predicted Reactivity | Rt, Bv, Or |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:100 | Hu, Ms |
| ICC | 1:500 | Hu |
Background
ATG12 protein is a crucial factor in the process of autophagy, serving as a human homolog of yeast autophagy-related genes. It plays an essential role in the formation of autophagosomes, the double-membrane structures that engulf and degrade cellular components. ATG12 forms a complex with ATG5 by being conjugated to it through the action of E1-like enzyme ATG7 and E2-like enzyme ATG10, and then this complex non-covalently binds with ATG16 to form the ATG12-ATG5-ATG16 complex, which is vital for the expansion of the autophagosome membrane. In terms of pathology, the expression of the ATG12 protein is associated with various diseases. For instance, reduced expression of ATG12 in cervical cancer tissue may be involved in the development of cervical cancer. Furthermore, the expression level of ATG12 correlates with the risk of recurrence after cervical conization surgery, with lower expression potentially increasing the risk of persistent or recurrent lesions.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti-ATG12 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti-ATG12 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human thyroid cancer. Anti-ATG12 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded mouse kidney. Anti-ATG12 antibody was used at 1/100 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC analysis of HeLa cells treated with chloroquine (50uM, 24 h) (top panel) and HeLa cells untreated with chloroquine (50uM, 24 h) (below panel). Anti- ATG12 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 100% ice-cold methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
