WB result of ASGPR1 Rabbit mAb
Primary antibody: ASGPR1 Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: Caco-2 whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 33 kDa
Observed MW: 40 kDa
ASGPR1 Recombinant Rabbit mAb (S-R292)
ASGPR1 Recombinant Rabbit mAb (S-R292)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ASGPR1 |
| Synonyms | Asialoglycoprotein receptor 1, ASGP-R 1, CLEC4H1 |
| Accession | P07306 |
| Clone Number | S-R292 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IP |
| Reactivity | Hu |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | null |
| IP | 1:50 | null |
Background
ASGPR1 is a subunit of the asialoglycoprotein receptor. This receptor is a transmembrane protein that plays a critical role in serum glycoprotein homeostasis by mediating the endocytosis and lysosomal degradation of glycoproteins with exposed terminal galactose or N-acetylgalactosamine residues. The asialoglycoprotein receptor may facilitate hepatic infection by multiple viruses including hepatitis B, and is also a target for liver-specific drug delivery.
Picture
Picture
Western Blot
IP
ASGPR1 Rabbit mAb at 1/50 dilution (1 µg) immunoprecipitating ASGPR1 in 0.4 mg HepG2 whole cell lysate.
Western blot was performed on the immunoprecipitate using ASGPR1 Rabbit mAb at 1/1000 dilution.
Secondary antibody (HRP) for IP was used at 1/400 dilution.
Lane 1: HepG2 whole cell lysate 20 µg (Input)
Lane 2: ASGPR1 Rabbit mAb IP in HepG2 whole cell lysate
Lane 3: Rabbit monoclonal IgG IP in HepG2 whole cell lysate
Predicted MW: 33 kDa
Observed MW: 46 kDa
