WB result of ASCL1/MASH1 Recombinant Rabbit mAb
Primary antibody: ASCL1/MASH1 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: U-2 OS whole cell lysate 20 µg
Lane 2: NCI-H69 whole cell lysate 20 µg
Negative control: U-2 OS whole cell lysate
Secondary antibody: Goat Anti- rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 25 kDa
Observed MW: 30 kDa
This blot was developed with high sensitivity substrate
ASCL1/MASH1 Recombinant Rabbit mAb (SDT-3517-121)
ASCL1/MASH1 Recombinant Rabbit mAb (SDT-3517-121)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | ASCL1/MASH1 |
| Synonyms | Achaete-scute homolog 1; ASH-1; hASH1; Class A basic helix-loop-helix protein 46 (bHLHa46); BHLHA46; HASH1; ASCL1 |
| Immunogen | Recombinant Protein |
| Location | Nucleus |
| Accession | P50553 |
| Clone Number | SDT-3517-121 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu |
| Positive Sample | NCI-H69 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:500-1:1000 | Hu |
| IHC-P | 1:250 | Hu |
| ICC | 1:500 | Hu |
Background
ASCL1 (Achaete-Scute Family BHLH Transcription Factor 1), also known as MASH1 (Mammalian Achaete-Scute Homolog 1), is a basic helix-loop-helix (bHLH) transcription factor that plays a pivotal role in neurogenesis and neuronal differentiation during embryonic development. Primarily expressed in the developing central and peripheral nervous systems, ASCL1 functions as a proneural factor that promotes the specification and differentiation of neural progenitor cells into various neuronal subtypes, including autonomic neurons, olfactory sensory neurons, and specific populations of interneurons in the forebrain. ASCL1 orchestrates complex transcriptional programs by binding to E-box motifs in target gene promoters, thereby activating expression of downstream effectors involved in cell cycle exit, neuronal migration, and axon guidance. In addition to its developmental functions, ASCL1 has gained significant attention in cancer biology, particularly as a master lineage-survival oncogene in neuroendocrine tumors, including small cell lung cancer (SCLC) and neuroblastoma, where it drives proliferation and maintains the neuroendocrine phenotype. Recent advances in cellular reprogramming have also leveraged ASCL1, in combination with other factors, to directly convert fibroblasts into induced neuronal cells, underscoring its potent neurogenic capacity and therapeutic potential for regenerative medicine applications.
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Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human small cell lung cancer. Anti-ASCL1/MASH1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human lung adenocarcinoma. Anti-ASCL1/MASH1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Negative control: IHC shows negative staining in paraffin-embedded human kidney. Anti-ASCL1/MASH1 antibody was used at 1/250 dilution, followed by a HRP Polymer for Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunocytochemistry
ICC shows positive staining in NCI-H69 cells (top panel) and negative staining in U-2 OS cells (below panel). Anti- ASCL1/MASH1 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. The cells were fixed with 4% PFA and permeabilized with 0.1% PBS-Triton X-100. Nuclei were counterstained with DAPI (Blue). Counterstain with tubulin (Red).
