WB result of Aquaporin 5/AQP5 Recombinant Rabbit mAb
Primary antibody: Aquaporin 5/AQP5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled KATO Ⅲ whole cell lysate 20 µg
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 24 kDa
Aquaporin 5/AQP5 Recombinant Rabbit mAb (S-1622-5)
Aquaporin 5/AQP5 Recombinant Rabbit mAb (S-1622-5)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Aquaporin 5/AQP5 |
| Synonyms | AQP-5 |
| Immunogen | Synthetic Peptide |
| Location | Cell membrane, Apical cell membrane |
| Accession | P55064 |
| Clone Number | S-1622-5 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Positive Sample | KATO Ⅲ, mouse lung, rat lung |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt |
| IHC-P | 1:500 | Hu |
| IF | 1:500 | Ms |
Background
Aquaporin 5 (AQP5) is a water channel protein that plays a significant role in the generation of saliva, tears, and pulmonary secretions. It is a member of the major intrinsic protein family, characterized by its ability to selectively transport water across cell membranes. AQP5 is predominantly expressed in the salivary and lacrimal glands, as well as in the respiratory tract, and is crucial for maintaining water homeostasis within these secretions. The protein is also implicated in the pathogenesis of certain diseases, such as Sjögren's syndrome, where its abnormal subcellular distribution in salivary gland cells may contribute to the disease's development.
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Western Blot
WB result of Aquaporin 5/AQP5 Recombinant Rabbit mAb
Primary antibody: Aquaporin 5/AQP5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled mouse kidney lysate 20 µg
Lane 2: unboiled mouse lung lysate 20 µg
Negative control: unboiled mouse kidney lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 24 kDa
WB result of Aquaporin 5/AQP5 Recombinant Rabbit mAb
Primary antibody: Aquaporin 5/AQP5 Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: unboiled rat kidney lysate 20 µg
Lane 2: unboiled rat lung lysate 20 µg
Negative control: unboiled rat kidney lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 28 kDa
Observed MW: 24 kDa
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human testis. Anti-Aquaporin 5/AQP5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-Aquaporin 5/AQP5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-Aquaporin 5/AQP5 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
Immunofluorescence
IF shows positive staining in paraffin-embedded mouse lung. Anti- Aquaporin 5/AQP5 antibody was used at 1/500 dilution (Green) and incubated overnight at 4°C. Goat polyclonal Antibody to Rabbit IgG - H&L (Alexa Fluor® 488) was used as secondary antibody at 1/1000 dilution. Counterstained with DAPI (Blue). Heat mediated antigen retrieval with EDTA buffer pH9.0 was performed before commencing with IF staining protocol.
