WB result of Apo-D Recombinant Rabbit mAb
Primary antibody: Apo-D Recombinant Rabbit mAb at 1/1000 dilution
Lane 1: HepG2 whole cell lysate 20 µg
Lane 2: SK-MEL-28 whole cell lysate 20 µg
Negative control: HepG2 whole cell lysate
Secondary antibody: Goat Anti-rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 21 kDa
Observed MW: 23 kDa
Apo-D Recombinant Rabbit mAb (S-1869-50)
Apo-D Recombinant Rabbit mAb (S-1869-50)
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Product Details
Product Details
Product Specification
| Host | Rabbit |
| Antigen | Apo-D |
| Synonyms | Apolipoprotein D; ApoD; APOD |
| Immunogen | Synthetic Peptide |
| Location | Secreted |
| Accession | P05090 |
| Clone Number | S-1869-50 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | SK-MEL-28 |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu |
| IHC-P | 1:500 | Hu |
Background
Apo-D, or apolipoprotein D, is a glycoprotein with a molecular weight of approximately 33 kDa, primarily produced in the brain and testes rather than the liver, which is typical for most lipoproteins. It is a component of high-density lipoprotein (HDL) and plays a crucial role in lipid metabolism by interacting with lecithin-cholesterol acyltransferase, an enzyme involved in the metabolism of lipoproteins. Apo-D is also implicated in various neurological disorders and is considered a biomarker for conditions such as androgen insensitivity syndrome and certain neurodegenerative diseases. Its function in the nervous system is thought to be related to neuroprotection and repair, possibly due to its antioxidant and anti-inflammatory properties.
Picture
Picture
Western Blot
Immunohistochemistry
IHC shows positive staining in paraffin-embedded human kidney. Anti- Apo-D antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human liver. Anti- Apo-D antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human breast cancer. Anti- Apo-D antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
