Flow cytometric analysis of Human CD31 expression on Human PBMC (human peripheral blood mononuclear cells). Human PBMC were stained with either APC Mouse IgG1, κ Isotype Control (Black line histogram) or SDT APC Mouse Anti-Human CD31 antibody (Red line histogram) at 0.1 μg/test, cells without incubation with primary antibody and secondary antibody (Blue line histogram) was used as unlabelled control. Fluorescent histograms were derived from gated events with the side and forward light-scattering characteristics of viable monocytes Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
APC Mouse Anti-Human CD31 Antibody (S-934-6)
APC Mouse Anti-Human CD31 Antibody (S-934-6)
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Product Details
Product Details
Product Specification
| Host | Mouse |
| Antigen | CD31 |
| Synonyms | PECAM-1; EndoCAM; GPIIA; PECA1 |
| Immunogen | Recombinant Protein |
| Location | Cell membrane |
| Accession | P16284 |
| Clone Number | S-934-6 |
| Antibody Type | Mouse mAb |
| Isotype | IgG1,k |
| Application | FCM |
| Reactivity | Hu |
| Positive Sample | Human PBMC |
| Purification | Protein G |
| Concentration | 0.02mg/ml |
| Conjugation | APC |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Hu |
Background
Cluster of differentiation 31 (CD31) also known as platelet endothelial cell adhesion molecule (PECAM-1) is a member of the immunoglobulin superfamily and is likely involved in leukocyte transmigration, angiogenesis, and integrin activation. PECAM-1 plays a key role in removing aged neutrophils from the body. PECAM-1 is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. In immunohistochemistry, CD31 is used primarily to demonstrate the presence of endothelial cells in histological tissue sections. This can help to evaluate the degree of tumor angiogenesis, which can imply a rapidly growing tumor. Malignant endothelial cells also commonly retain the antigen, so that CD31 immunohistochemistry can also be used to demonstrate both angiomas and angiosarcomas. It can also be demonstrated in small lymphocytic and lymphoblastic lymphomas, although more specific markers are available for these conditions.
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