Flow cytometric analysis of Mouse Ly-6G/Ly-6C(Gr-1) expression on C57BL/6 mouse bone marrow. C57BL/6 mouse bone marrow were stained with Brilliant Violet 510™ Rat Anti-Mouse CD11b antibody and either APC-Cy7 Rat IgG2b, κ Isotype Control (Left panel) or SDT APC-Cy7 Rat Anti- Mouse Ly-6G/Ly-6C(Gr-1) antibody (Right panel) at 0.1 μg/test. Flow cytometry and data analysis were performed using BD FACSymphony™ A1 and FlowJo™ software.
APC-Cy7 Rat Anti-Mouse Ly-6G/Ly-6C(Gr-1) Antibody (S-R425)
APC-Cy7 Rat Anti-Mouse Ly-6G/Ly-6C(Gr-1) Antibody (S-R425)
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Product Details
Product Details
Product Specification
| Host | Rat |
| Antigen | Ly-6G/Ly-6C(Gr-1) |
| Synonyms | Lymphocyte antigen 6G; Ly-6G.1 |
| Location | Cell membrane |
| Accession | P35461 |
| Clone Number | S-R425 |
| Antibody Type | Rat mAb |
| Isotype | IgG2b,k |
| Application | FCM |
| Reactivity | Ms |
| Positive Sample | C57BL/6 mouse bone marrow |
| Purification | Protein G |
| Concentration | 0.02mg/ml |
| Conjugation | APC-Cy7 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 5 μl per million cells in 100μl volume | Ms |
Background
Ly-6G protein is a GPI (glycosylphosphatidylinositol)-anchored protein. Ly-6G is primarily expressed on monocytes, neutrophils, and T cells. It plays a crucial role in inhibiting anti-tumor efficacy and T-cell responses. Due to its specific expression on neutrophils, Ly-6G has been widely used as a surface marker in flow cytometry, cell sorting, and immune phenotyping experiments. Unique expression of Ly-6G in bone marrow indicates its involvement in hematopoietic microenvironment, potentially regulating the development or function of immune cells within the bone marrow niche.
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